biological source
human blood
packaging
tube of 5 μg 92110206-DNA-5UG, pkg of vial of cells 92110206-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Surface Immunoglobulin ( Ig), MHC Class II
receptors
Fc, complement
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
T cell replacement factor (TRF) assays, B cell differentiation studies
Biochem/physiol Actions
Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.
Human lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17
Preparation Note
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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