生物来源
hamster lung
包装
tube of 5 μg 93010723-DNA-5UG
pkg of vial of cells 93010723-1VL
生长模式
Adherent
核型
2n = 22
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Hamster Chinese lung
细胞系描述
Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).
应用
Mutagenesis and mammalian cell genetic studies
培养基
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm2; 5% CO2; 37°C using 0.25% trypsin/EDTA.
其他说明
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