生物来源
fish skin
包装
tube of 5 μg 93120820-DNA-5UG
pkg of vial of cells 93120820-1VL
生长模式
Adherent
核型
2n = 96
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
相关类别
细胞系来源
Fish epithelioma Fathead minnow Pimephales promelas
细胞系描述
This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.
DNA图谱分析
Not specified
培养基
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
其他说明
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