WIN human cell line

Consanguineous: No

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

A: 101
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQA1: 201
HLA DQB1: 0201 03032
HLA DPA1: 01 0201
HLA DPB1: 0401 1301

HLA A: 1
HLA A ief: 1
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2 9
HLA DP: 4

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.