生物来源
human blood
包装
tube of 5 μg 96090512-DNA-5UG
pkg of vial of cells 96090512-1VL
生长模式
Suspension
核型
92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
形态学
Lymphoblastoid
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human Caucasian B-cell lymphoma
细胞系描述
The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.
应用
Study of B-cell lymphoma-leukaemia
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.
其他说明
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