packaging
pkg of 1 kit
Quality Level
technique(s)
UV/Vis spectroscopy: suitable, protein quantification: suitable
Amax
570 nm
application(s)
cell analysis
detection
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
General description
细胞生长测定试剂盒（基于MTT）设计用于以分光光度法测量细胞生长，作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法，可通过线粒体脱氢酶活性测量活细胞活性。
Application
细胞生长测定试剂盒（基于MTT）已用于：
- 测定成骨前细胞的细胞活力
- 正常人前列腺间质和上皮细胞的MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯溴化四唑)检测
- 测定原代宫颈上皮细胞的细胞活力
Packaging
每个试剂盒包括5瓶试剂，每瓶含1 mL 5 mg/ml MTT的RPMI溶液（无酚红）和50 mL MTT溶剂（0.1N盐酸的无水异丙醇溶液）。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加，因此每个试剂盒的测试次数取决于培养容量和/或体积大小。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
target_organs
Central nervous system
存储类别
3 - Flammable liquids
flash_point_f
53.6 °F - closed cup
flash_point_c
12 °C - closed cup
wgk
WGK 3
法规信息
新产品
此项目有
商品
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
相关内容
Datasheet
Mariarita Perri et al.
Biochimica et biophysica acta, 1800(9), 993-1001 (2010-07-06)
Vitamin A is suggested to be protective against oxidative stress. However, different authors observed pro-oxidant effects of retinoids both in experimental works and clinical trials. These discordances are the bases for the investigation of the proliferative and anti-proliferative properties of
Shang-Long Liu et al.
Oncology letters, 17(2), 2057-2062 (2019-01-25)
The biological features of pancreatic cancer and the associated hypoxic environment around the cancer cells often lead to resistance to radiotherapy and chemotherapy. The present study was performed in order to explore the effect pancreatic stellate cells (PSCs) have on
Abdullah Maha et al.
Hematology (Amsterdam, Netherlands), 13(1), 13-20 (2008-06-07)
Despite the advances in understanding the pathophysiology of acute myeloid leukaemia (AML), the cure rate for acute myeloid leukaemia patients remains low. Cytogenetic abnormalities and age are the prognostic factors that guide treatment decisions. However, many AML patients still die.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CGD1-1KT | 04061833531747 |