F3040
抗FLAG® M1单克隆抗体 小鼠抗
clone M1, purified immunoglobulin, buffered aqueous solution
别名:
抗 ddddk, 抗 dykddddk
biological source
(Bioreactor)
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M1, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot: 10 μg/mL
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。
纯化方法-蛋白A
纯化方法-蛋白A
Immunogen
FLAG；肽序列 DYKDDDDK
Application
抗-FLAG® M1单克隆抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹和EIA分析。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
更多产品信息，请访问我们的FLAG®应用页面。
成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀（1 篇文章）
免疫荧光（1 篇文章）
更多产品信息，请访问我们的FLAG®应用页面。
Biochem/physiol Actions
当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合，因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性；该复合物在不存在钙离子的情况下解离。
Physical form
溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。
Preparation Note
将抗体稀释至 10ug/mL，置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。
Other Notes
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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