G9295
单克隆抗 GAPDH-过氧物酶 小鼠抗
clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution
别名:
抗 G3PD, 抗 G3PDH, 抗 甘油醛-3-磷酸脱氢酶
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
peroxidase conjugate
Clone:
GAPDH-71.1, monoclonal
Application:
WB
Citations:
234
Quality Level
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GAPDH-71.1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~37 kDa
species reactivity
rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
should not react with
prokaryotes
technique(s)
western blot: 1:35,000-1:70,000 using A431 total cell extract
isotype
IgM
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)
General description
单克隆抗GAPDH（小鼠IgM同种型）源自通过小鼠骨髓瘤细胞（NS1细胞）融合产生的杂交瘤GAPDH-71.1和通过兔GAPDH免疫的BALB/c小鼠脾细胞。
甘油醛-3-磷酸脱氢酶（GAPDH）的基因定位于人染色体12p13。该蛋白定位于细胞质中，但可根据细胞条件转移至细胞核。
Immunogen
兔 GAPDH。
Application
小鼠抗GAPDH-过氧化物酶单克隆抗体还可用于
- 免疫印迹
- 酶联免疫吸附测定（ELISA）
- 免疫细胞化学
Biochem/physiol Actions
甘油醛-3-磷酸脱氢酶（GAPDH）是含有相同链的四聚体。它可催化甘油醛-磷酸盐的可逆氧化磷酸化，其碳水化合物代谢中的关键能量产生步骤。它可与几种蛋白质结合，包括肌动蛋白、微管蛋白、淀粉样蛋白前体、聚谷氨酰胺肽、DRPLA（齿状核红核苍白球丘脑下部核萎缩）和亨廷顿蛋白。蛋白激酶Cι/λ可结合并磷酸化GAPDH。磷酸化的GAPDH可与细胞骨架元件结合并控制早期分泌途径中的微管动力学。聚（ADP-核糖）聚合酶-1（PARP1）可与GAPDH相互作用，从而在氧化/亚硝化应激的存在下介导脑损伤。GAPDH形成OCA-S的一部分，其中OCA-S是多组分OCT1（八聚体-基序-结合因子）共激活因子复合物，其可参与S期依赖性组蛋白H2B的转录。该关联可将H2B转录机制与细胞周期调节和细胞代谢状态联系起来。GAPDH也是功能性GAIT（干扰素γ激活的翻译抑制剂）mRNP（信使核糖核蛋白）的组分。在黑素瘤进展期间GAPDH表达失调。
该抗体不与非脊椎动物和原核生物 GAPDH 发生交叉反应。
Physical form
溶解在pH7.4、含有0.05%MIT的0.01M磷酸缓冲盐。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或人类或动物应用。
未找到合适的产品？
试试我们的产品选型工具.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Heterogeneous cadherin expression and multicellular aggregate dynamics in ovarian cancer dissemination
Klymenko Y, et al.
Neoplasia, 19(7), 549-563 (2017)
Muscle Lim Protein and myosin binding protein C form a complex regulating muscle differentiation
Arvanitis DA, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1864(12), 2308-2321 (2017)
Bothrops jararaca venom metalloproteinases are essential for coagulopathy and increase plasma tissue factor levels during envenomation
Yamashita KM, et al.
PLoS Neglected Tropical Diseases, 8(5), e2814-e2814 (2014)
Jennifer L Whiting et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(30), E4328-E4337 (2016-07-13)
Filtration through the kidney eliminates toxins, manages electrolyte balance, and controls water homeostasis. Reabsorption of water from the luminal fluid of the nephron occurs through aquaporin-2 (AQP2) water pores in principal cells that line the kidney-collecting duct. This vital process
Naoya Yuhki et al.
Genome research, 13(6A), 1169-1179 (2003-05-14)
To study comparative molecular dynamics in the genesis of the major histocompatibility complex (MHC), we determined a complete nucleotide sequence spanning 758,291 bp of the domestic cat (Felis catus) extended and classical class II region. The feline class II MHC
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持