Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
McCoy 5A培养基推荐用于在原代细胞培养中生产病毒。
Application
McCoy 5A培养基已用于培养：
- 人结肠癌细胞系（HCT116、RKO、FET、CBS、HCT116b和TENN），以研究富亮氨酸重复G蛋白偶联受体（LGR5）在结肠癌转移中的作用
- 人结肠HCT116细胞，以研究miR-224在肿瘤中的过表达
- 成骨细胞，以研究缺钙羟基磷灰石在标准细胞培养基中的离子反应性
Biochem/physiol Actions
McCoys 5A培养基起初是通过修改BME中的氨基酸，用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。
Preparation Note
按照每升培养基11.9克粉末配制。
补充2.2 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
监管及禁止进口产品
此项目有
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
商品
McCoy's 5A Modified Media Formulation
相关内容
Instructions
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M4892-10L | 04061835568833 |
| M4892-10X1L | 04061835568840 |
| M4892-1L | 04061837473593 |