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M8883

4-甲基伞形酮磷酸酯

Name: 4-甲基伞形酮磷酸酯
Brand: SIGMA

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

别名:

4-甲基伞形基磷酸

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关于此项目

经验公式（希尔记法）:

C₁₀H₉O₆P

化学文摘社编号:

3368-04-5

分子量:

256.15

NACRES:

NA.32

PubChem Substance ID:

24897292

UNSPSC Code:

12352204

EC Number:

222-137-7

MDL number:

MFCD00016969

Beilstein/REAXYS Number:

1687229

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属性

产品名称

4-甲基伞形酮磷酸酯, phosphatase substrate

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL, clear to very slightly hazy, colorless

fluorescence

λ_ex 319 nm; λ_em 384 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

说明

General description

磷酸酶的荧光底物。

Application

4-甲基伞形酮磷酸酯可在肽结合试验中作为碱性磷酸酶的作用底物。也用作酶联免疫吸附测定(ELISA)的荧光底物。

Biochem/physiol Actions

4-甲基伞形酮磷酸酯可作为钙调蛋白依赖性磷酸酶的荧光底物，也可用于碱性磷酸酶的动力学研究。在酶联免疫吸附测定(ELISA)中，可作为碱性磷酸酶作用底物。在人免疫缺陷型病毒抗体的酶免疫分析中，作为底物的4-甲基伞形酮磷酸酯比酚酞单磷酸酯灵敏7倍，比对硝基苯磷酸酯灵敏8-13倍。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Highly sensitive active-site titration of lipase in microscale culture media using fluorescent organophosphorus ester.

Ryota Fujii et al.

Biochimica et biophysica acta, 1631(2), 197-205 (2003-03-14)

The fluorescent organophosphorus esters, diethyl 4-methylumbelliferyl phosphate (1), ethyl hexyl 4-methylumbelliferyl phosphate (2) and ethyl 4-methylumbelliferyl heptylphosphonate (3) have been synthesized and evaluated as a sensitive active-site titrant of lipase. The phosphorus esters 1, 2 and 3 inactivated the lipase

Recognition of human proinsulin leader sequence by class I-restricted T-cells in HLA-A*0201 transgenic mice and in human type 1 diabetes.

Andréa Toma et al.

Diabetes, 58(2), 394-402 (2008-11-18)

A restricted region of proinsulin located in the B chain and adjacent region of C-peptide has been shown to contain numerous candidate epitopes recognized by CD8(+) T-cells. Our objective is to characterize HLA class I-restricted epitopes located within the preproinsulin

Association of Intestinal Alkaline Phosphatase With Necrotizing Enterocolitis Among Premature Infants.

Maya Heath et al.

JAMA network open, 2(11), e1914996-e1914996 (2019-11-09)

Necrotizing enterocolitis (NEC) in preterm infants is an often-fatal gastrointestinal tract emergency. A robust NEC biomarker that is not confounded by sepsis could improve bedside management, lead to lower morbidity and mortality, and permit patient selection in randomized clinical trials

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