usage
sufficient for 200 tests (UV)
feature
This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer
storage temp.
−20°C
General description
酮体 3-羟基丁酸 (BOH) 和乙酰乙酸 (AcAc) 主要在肝脏中由脂肪酸氧化生成 ，正常情况下在尿液和血液中浓度较低。血液中酮体浓度升高可能导致代谢性酸中毒，这与糖尿病、儿童期低血糖、生长激素缺乏症、酒精或水杨酸盐中毒以及先天性代谢缺陷相关。
Application
可用于检测血清、血浆、尿液及其他生物样品中的酮体（乙酰乙酸和3-羟基丁酸）。
酮体测定法已用于测量人肝癌细胞系 HepG2 培养基中酮体的释放。
Biochem/physiol Actions
在本试剂盒中，使用基于 3-羟基丁酸脱氢酶催化反应的酶分析法测定 AcAc 和 BOH 水平，其中在 340 nm 处测定的 NADH 吸光度更改与 AcAc 和 BOH 浓度直接相关。
Features and Benefits
兼容高通量的操作系统。
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
常规特殊物品
此项目有
相关内容
Instructions
Kim J Shimy et al.
Journal of the Endocrine Society, 4(7), bvaa062-bvaa062 (2020-07-16)
According to the carbohydrate-insulin model of obesity, an elevated insulin-to-glucagon ratio in response to a high-carbohydrate diet directs metabolic fuels toward storage, resulting in lower circulating energy. To determine differences in total circulating energy post-meal related to dietary carbohydrate. Ancillary
Hyperketonemia and ketosis increase the risk of complications in type 1 diabetes.
Kanikarla M, et al.
Free Radical Biology & Medicine, 95, 268-277 (2016)
Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.
Dedkova E N and Lothar A B
Frontiers in Physiology, 5, 260-260 (2014)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAK134-1KT | 04061834065494 |