input
cell culture medium
plasma
serum
urine
detection method
fluorometric
relevant disease(s)
diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
storage temp.
−20°C
Quality Level
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Application
The L-Lactate Assay Kit may be used for:
- Lactate Metabolism Research
- Tissue Metabolism Research
- Drug Discovery
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
Biochem/physiol Actions
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
Features and Benefits
- Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
- Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
- High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
相关内容
Instructions
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