MBD0052
Hemin solution
Ready-made microbial growth medium supplement, 0.5 g/L
别名:
Chlorohemin solution, ChloroprotoporphyrinIX iron(III) solution, Ferriprotoporphyrin IX chloride Solution
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关于此项目
NACRES:
NA.21
UNSPSC Code:
41106212
Quality Level
form
liquid
usage
supplement for growth media
concentration
0.5 g/L
shipped in
wet ice
storage temp.
2-8°C
General description
Hemin is a pro-oxidant, hemolysis derived-product. It is essential for the growth of heme-deficient organisms such as trypanosomatids. Hemin readymade solution is used as a culture-medium supplement for fastidious anaerobic microorganisms, shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms. It is present as a key ingredient compound in common anaerobic growth media, such as Brucella broth, Thioglycolate broth and Reinforced Clostridial Medium.
Application
Hemin solution may be used as a supplement in brain heart infusion-yeast extract-hemin (BHI-YH) medium to determine cholesterol-to-coprostanol reducing activity.
Features and Benefits
- Convenient readymade solution to speed up media preparation
- Sterile solution
- Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples
Other Notes
- For 100 ml of medium preparation, it is recommended to add 1 mL hemin ready-made solution after autoclaving the medium, to achieve a final concentration of 5 microgram/ml. The optimal concentration of the supplement may vary for each strain.
- The solution is classified as light sensitive. 2. The solution should not be heated and must be added aseptically to autoclaved media cooled to 50-55°C. 3.
- Select strains which show turbid growth (24-48 hr.) with Hemin supplementation: Porphyromonas gingivalis, Bifidobacterium longum, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
Manual of clinical microbiology
Murray, P.R., E.J. Baron, M.A.Pfaller, F.C. Tenover, and R.H.Yolken
ASME 2013 Gas Turbine India Conference. American Society of Mechanical Engineers, 2013., 2, 94305-95250 (2006)
María Elisa Lombardo et al.
Analytical biochemistry, 341(2), 199-203 (2005-05-24)
Hemin chlorides exhibit two absorption maxima in the Soret region, one at about 360-380 nm (S' band) and the other between 400 and 430 nm (S band). We present here a simple and fast spectrophotometric assay to determine concentration of
Sânzio Silva Santana et al.
Frontiers in immunology, 11, 1488-1488 (2020-08-09)
The excessive release of heme during hemolysis contributes to the severity of sickle cell anemia (SCA) by exacerbating hemoglobin S (HbS) autoxidation, inflammation and systemic tissue damage. The present study investigated the effect of hydroxyurea (HU) on free radical neutralization