grade
Molecular Biology
Quality Level
form
liquid
feature
Direct PCR
technique(s)
DNA amplification: suitable, DNA extraction: suitable, PCR: suitable
input
crude DNA
storage temp.
−20°C
General description
这种中和缓冲液B要与 Sigma′REDExtract-N-Amp™ 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液，并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B，每次提取至少可中和一次。 不过，如果需要更多的扩增反应，则可能需要补充额外的中和缓冲液。
Application
中和缓冲液B 已用于改良 Sigma Extract-N-Amp™提取程序。它还用于小鼠的基因分型，在聚合酶链反应 (PCR) 之前可使用Sigma的REDExtract-N-Amp™ PCR 反应混合物来中和DNA提取液。
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
法规信息
低风险生物材料
此项目有
实验方案
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N3910-2.5ML | 04061837301117 |
| N3910-240ML | 04061837301131 |
| N3910-6ML | 04061837301186 |
| N3910-1.2ML | 04061837301094 |
| N3910-60ML | 04061837301162 |
| N3910-24ML | 04061834116387 |