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Merck
CN

R1755

罗丹明 B 异硫氰酸酯

BioReagent, mixed isomers, suitable for protein labeling

别名:

RBITC

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关于此项目

经验公式（希尔记法）:
C29H30ClN3O3S
化学文摘社编号:
36877-69-7
分子量:
536.08
NACRES:
NA.32
PubChem Substance ID:
329824007
UNSPSC Code:
12352116
EC Number:
253-248-9
MDL number:
MFCD00136007

主要文件

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InChI key

YVSWPCCVTYEEHG-UHFFFAOYSA-N

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

description

labeling efficiency with bovine albumin > 70%

product line

BioReagent

Quality Level

200

form

powder

mol wt

536.08 g/mol

composition

9-[2-carboxy-5(or 6)-isothiocyanatophenyl]-3,6-bis(diethylamino)xanthylium chloride, ≤100%

color

purple to red-brown

solubility

methanol: 10 mg/mL (dark red to very dark red and dark purple to very dark purple)

fluorescence

λex 543 nm; λem 580 nm in methanol

suitability

suitable for protein labeling

storage temp.

−20°C

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相关类别

General description

罗丹明 B 异硫氰酸酯（RhBITC）是一种有机荧光染料，在各种生物应用中，广泛地用作荧光标签。RhBITC 吸附在二氧化硅或聚苯乙烯珠粒的Ag 上。它不仅表现出强烈的表面增强拉曼散射（SERS）信号，还表现出可测量的荧光量。RhBITC 改性的Ag 沉积二氧化硅或聚苯乙烯珠粒在乙醇中能很好地分散。它们也易于在水中被聚电解质包被，以便进一步衍生出可与靶分子相结合的目标生物分子。

Application

罗丹明 B 异硫氰酸酯用于以下应用：
  • 罗丹明异硫氰酸酯标记
  • 罗丹明标记的合成 APF
  • 蛋白质和荧光结合物的制备
  • 荧光红染料

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SHBS8871
SHBR3111
SHBN1684
SHBM7514
SHBM0054

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Control Over Aragonite Crystal Nucleation and Growth: An In Vitro Study of Biomineralization.
Yael Levi et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 4 (1998)
Kwan Kim et al.
Biosensors & bioelectronics, 24(7), 1864-1869 (2008-11-05)
Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads
S K Williams et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(4), 2393-2397 (1981-04-01)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of
Jun-Sung Kim et al.
Journal of veterinary science, 7(4), 321-326 (2006-11-16)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could
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