R3155
Monoclonal Anti-Rabbit IgG, Native−Peroxidase 小鼠抗
clone RabT-50, purified from hybridoma cell culture
别名:
Mouse Anti-Rabbit IgG
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
RabT-50, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
14
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RabT-50, monoclonal
form
buffered aqueous solution
species reactivity
rabbit
should not react with
human, horse, bovine, cat, rat, chicken, pig, goat, monkey
concentration
2.5-5.0 mg/mL (antibody)
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.
Immunogen
Enriched fraction of rabbit IgG light chain.
Application
Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- western blotting
Biochem/physiol Actions
Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
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