SAB4500028
Anti-AOS1 antibody produced in rabbit
affinity isolated antibody
别名:
AOS1, SAE1, SUA1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IF, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 38 kDa
species reactivity
rat, mouse, human
concentration
~1 mg/mL
technique(s)
ELISA: 1:20000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SAE1(10055)
General description
E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle.
Immunogen
The antiserum was produced against synthesized peptide derived from human AOS1.
Immunogen Range: 221-270
Immunogen Range: 221-270
Biochem/physiol Actions
SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
SUMO-activating SAE1 transcription is positively regulated by Myc.
Amente S, et al.
American Journal of Cancer Research, 2(3), 330-334 (2012)
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.
Tatham MH, et al.
The Journal of Biological Chemistry, 276(38), 35368-35374 (2001)
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, et al.
Faseb Journal, 15(10), 1825-1827 (2001)
Robert Coukos et al.
eLife, 10 (2021-08-21)
The trafficking of specific protein cohorts to correct subcellular locations at correct times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach to probe the molecular mechanisms of protein trafficking, but
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