recombinant
expressed in HEK 293 cells
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RM179, monoclonal, recombinant monoclonal
form
buffered aqueous glycerol solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
ELISA: 0.5-1 μg/mL, immunocytochemistry: 0.5-2 μg/mL, immunohistochemistry: 1 ug/mL – 10 ug/mL, western blot: 1-2 μg/mL
isotype
IgG
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
acetylation (Lys56)
Quality Level
Gene Information
human ... H3C1(8350)
General description
哺乳动物主要包括三类组蛋白H3变体:复制组蛋白(H3.1 和 H3.2)、替换组蛋白 (H3.3) 和着丝粒组蛋白 (Cenp-A)。组蛋白H3位于人类染色体 6p22 上。
Immunogen
对应于乙酰化组蛋白H3(Lys56)的合成肽
Biochem/physiol Actions
组蛋白是染色质的基本构成单元。成人小脑高等级神经胶质瘤中的组蛋白H3 K27M突变。它可控制蛋白质与蛋白质的相互作用,以诱导驱动染色质凝聚的反式作用因子结合。
该抗体可与在56号赖氨酸乙酰化的组蛋白H3(K56ac)发生反应。不与组蛋白H3中其他乙酰化的赖氨酸具有交叉反应性。
Features and Benefits
完放心地使用我们的抗体。如果抗体在您的申请的研究中不起作用,我们将全额退款或安排替代抗体。了解更多信息。
Physical form
溶于含有50%甘油、1%BSA和0.09%叠氮化钠的磷酸盐缓冲盐水中。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Nataliya Danilenko et al.
Nature communications, 10(1), 3435-3435 (2019-08-08)
Histones, the principal protein components of chromatin, contain long disordered sequences, which are extensively post-translationally modified. Although histone chaperones are known to control both the activity and specificity of histone-modifying enzymes, the mechanisms promoting modification of highly disordered substrates, such
Signaling to chromatin through histone modifications.
Cheung P, et al.
Cell, 103(2), 263-271 (2000)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34(3), 113-119 (2017)
The program for processing newly synthesized histones H3.1 and H4.
Campos EI
Nature Structural and Molecular Biology, 17(11), 1343-1351 (2010)
A comprehensive view of the epigenetic landscape part I: DNA methylation, passive and active DNA demethylation pathways and histone variants.
Sadakierska-Chudy A
Neurotoxicity Research, 27(1), 84-97 (2015)
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