description
wavelength 500-750nm
Quality Level
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature, 200 μL sample volume
storage temp.
2-8°C
General description
双缩脲和 Lowry 程序用于蛋白质测定。前者广泛用于临床检测。后者尽管更灵敏，却只用于研究工作，受限于组合试剂的低稳定性、颜色的不可再现性（特别是在低蛋白浓度下）以及和蛋白质浓度的非线性显色反应。Ohnishi 和 Barr 改良了用于 Lowry 测定法的双缩脲试剂，从而简化了流程，同时提高了组合试剂的稳定性。
Application
全蛋白试剂盒（ Micro-Lowry, Onishi & Barr Modification ）已用于测定以下物质的总可溶蛋白质浓度:
- 麦醇溶蛋白的透析液/提取物
- 面粉（燕麦 和 硬粒小麦）和种子（藜麦, 芡欧鼠尾草）的提取物
- 储存的大鼠肝微粒体部分样品
Biochem/physiol Actions
比色法，终点。 化验。Ohnishi 和 Barr′s 对微 Lowry 方法的改进。根据实验步骤，稀释的缩二脲试剂与肽键反应生成紫蓝色复合物，通过加入苯酚试剂增强其颜色。在 550-750 nm 处读取的吸光度用于确定标准曲线的结果。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书 & 价格
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
易制毒化学品（2类）
此项目有
相关内容
Instructions
A Kaliszewska et al.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| TP0200-1KT | 04061837398001 |