V4388
Anti-VP16 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti Alpha trans-inducing protein
target post-translational modification
unmodified
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Immunogen
synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue.
Application
Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins. It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Jeremy R Teuton et al.
Journal of virology, 81(8), 3731-3739 (2007-01-19)
Herpes simplex virus type 1 (HSV-1) envelope proteins are posttranslationally modified by the addition of sialic acids to the termini of the glycan side chains. Although gC, gD, and gH are sialylated, it is not known whether sialic acids on
Ida G Lunde et al.
The Journal of physiology, 582(Pt 3), 1277-1287 (2007-04-28)
The effects of exercise on skeletal muscle are mediated by a coupling between muscle electrical activity and gene expression. Several activity correlates, such as intracellular Ca(2+), hypoxia and metabolites like free fatty acids (FFAs), might initiate signalling pathways regulating fibre-type-specific
Health care's future.
R J Nowak
Ohio medicine : journal of the Ohio State Medical Association, 87(5), 229-230 (1991-05-01)
Ying-Hsiu Su et al.
Journal of virology, 80(23), 11589-11597 (2006-09-22)
Following infection, the physical state of linear herpes simplex virus (HSV) genomes may change into an "endless" or circular form. In this study, using Southern blot analysis of the HSV genome, we provide evidence that immediate-early protein ICP4 is involved
Saranya Kittanakom et al.
Biochemical and biophysical research communications, 445(4), 746-756 (2014-02-25)
G-protein coupled receptors (GPCRs) are involved in a variety of disease processes and comprise major drug targets. However, the complexity of integral membrane proteins such as GPCRs makes the identification of their interacting partners and subsequent drug development challenging. A
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