Anti-Glucagon Antibody, clone 13D11.33 ZooMAb^® Mouse Monoclonal

Pro-glucagon (UniProt: P01275) is encoded by the GCG gene (Gene ID: 2641) in human. Pro-glucagon is a 180 amino acid peptide that is post-translationally processed in a tissue-specific manner in pancreatic α cells and intestinal L cells. In pancreatic α cells, glucagon (aa 53-81) is produced by cleavage of pro-glucagon by proprotein convertase subtilisin/Kexin type 2 (PCSK2/PC2). Glucagon is secreted in response to low blood glucose level. Hence, its circulating levels are low in the prandial state and increase 2-3 fold in response to fasting or hypoglycemia. It maintains glucose homeostasis by stimulating hepatic glucose production.  Its actions are mediated via the glucagon receptor (GCGR), a G protein-coupled receptor that is most abundantly expressed in the liver. Traces of GCGR are also reported in several extrahepatic tissues, such as the brain, heart, kidney, gastrointestinal tract, and adipose tissues.  It regulates blood glucose levels by increasing gluconeogenesis and reducing glycolysis. As a counterregulatory hormone of insulin, glucagon raises plasma glucose levels in response to insulin-induced hypoglycemia. In diabetic subjects, it plays a role in initiating and maintaining hyperglycemic condition. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Habegger, KM., et al. (2010). Nat. Rev. Endocrinol. 6(12); 689-697; Jiang, G., and Zhang, BB. (2003). Am. J. Physiol. Endocrinol. Metab. 284(4); E671-678).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Ovalbumin-conjugated full-length human Glucagon.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Glucagon in Human pancreas tissue sections.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Glucagon in rat pancreas tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Glucagon in Human pancreas tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Glucagon in Human Pancreas.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Glucagon in RIN-mF5 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 13D11.33 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects glucagon.

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.