biological source
rabbit
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SIG2-AC2, monoclonal
mol wt
antigen ~42 kDa
species reactivity
chicken, rat, human
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:400, western blot: 1:50-1:100 using chicken gizzard total extract
isotype
IgG
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
General description
Monoclonal Anti-Actin (20-33) (rabbit IgG) is a rabbit monoclonal derived from the hybridoma SIG2-AC2 produced by the fusion of rabbit myeloma cells and splenocytes from rabbits immunized with a synthetic peptide. Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds with at least six isoforms characterized by electrophoresis and amino acid sequence analysis. There are three α-actins (skeletal, cardiac and smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and g-non-muscle).
Immunogen
synthetic peptide corresponding to amino acids 20-33 of actin, attached to a multiple antigen peptide (MAP) backbone.
Application
Monoclonal Anti-Actin (20-33) antibody produced in rabbit has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunocytochemistry
Physical form
Supplied as ascites fluid containing 15 mM sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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