biological source
mouse
recombinant
expressed in mouse cell line
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
156215, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: 0.5-1.0 μg/mL, western blot: 1-2 μg/mL
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... ANGPT4(51378)
General description
Angiopoietin 4 is an endothelial cell-specific protein that is a ligand for the TIE2 tyrosine receptor. Angiopoietin 4 is critical for mature blood vessel formation.
Angiopoietins are growth factors vital for vascular differentiation during normal development. There are four angiopoietins discovered that bind to receptor tyrosine kinases Tie2. Human Angiopoietin-4 (ANG-4) expression is observed in lung and cultures HUVEC cells. Angiopoietin-4 mediates angiogenesis and migration of cells and is overexpressed in colorectal and gastric adenocarcinomas and glioblastomas. The cellular functions of ANG-4 are mediated by Akt and ERK1/2 pathways. The roles of ANG-3 and ANG-4 are significant in the survival of tumor cells, enhance angiogenesis and increase vascular branching. Reports also show that ANG-4 inhibits the angiogenesis in human small cell lung cancer cell line
Anti-Angiopoietin-4 detects human angiopoietin-4. The antibody shows no cross-reactivity with recombinant human angiopoietin-1, recombinant human angiopoietin-2, recombinant human angiopoietin-X, and recombinant human AngPTL3.
Anti-Angiopoietin-4 detects human angiopoietin-4. The antibody shows no cross-reactivity with recombinant human angiopoietin-1, recombinant human angiopoietin-2, recombinant human angiopoietin-X, and recombinant human AngPTL3.
Immunogen
purified, NSO-derived, recombinant human ANG-4.
Application
Anti-Angiopoietin-4 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA, the recommended concentration is 0.5-1.0 μg/ml.
Physical form
Monoclonal Anti-Angiopoietin-4 is supplied as approximately 500 μg of antiserum lyophilized from a 0.2 μm filtered solution of phosphate buffered saline with 5% trehalose.
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
相关内容
Datasheet
Shigetomo Fukuhara et al.
Histology and histopathology, 25(3), 387-396 (2010-01-08)
Angiopoietin (Ang) 1 is a ligand for endothelium-specific receptor tyrosine kinase Tie-2. In adult vasculature, Ang1/Tie2 signaling is thought to regulate both maintenance of vascular quiescence and promotion of angiogenesis. However, it has been unknown how Tie2 signal regulates these
Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
Melissa K Brunckhorst et al.
Cancer research, 70(18), 7283-7293 (2010-09-09)
Glioblastoma multiforme (GBM) is a highly invasive and vascularized aggressive brain tumor. Less than 10% of GBM patients survive >5 years after diagnosis. Angiogenesis plays an important role in GBM growth, and antiangiogenesis-based therapies have shown clinical efficacy for GBM
D M Valenzuela et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(5), 1904-1909 (1999-03-03)
The angiopoietins have recently joined the members of the vascular endothelial growth factor family as the only known growth factors largely specific for vascular endothelium. The angiopoietins include a naturally occurring agonist, angiopoietin-1, as well as a naturally occurring antagonist