A0855
抗植物生长素抗体，小鼠单克隆 小鼠抗
clone 1E11-C11, purified from hybridoma cell culture
别名:
抗 IAA, 抗 吲哚-3-乙酸
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关于此项目
Conjugate:
unconjugated
Clone:
1E11-C11, monoclonal
Application:
DB, ELISA (i), IF, IHC, WB
Citations:
15
biological source
mouse
recombinant
expressed in mouse cell line
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1E11-C11, monoclonal
form
PBS solution
species reactivity
plant
technique(s)
dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA, western blot: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
生长素是一种植物激素，调节生理生长和发育。该激素调节细胞功能，如伸长、膨压、分裂和分化。吲哚乙酸（IAA）被认为是多种植物中的主要生长素。IAA由色氨酸（Trp）通过Trp依赖性途径合成。小鼠单克隆抗生长素抗体识别吲哚乙酸，但是不识别游离的非甲基化IAA。
Immunogen
与卵清蛋白相连的吲哚乙酸 (IAA) 羧基。
Application
小鼠单克隆抗生长素抗体已用于免疫荧光分析。该抗体还可用于ELISA 、斑点印迹和蛋白质印迹分析等应用。
用单克隆抗吲哚-3-乙酸抗体通过免疫组织化学鉴定了正在发育的冷杉木 云杉植物石蜡包埋的福尔马林固定胚中的吲哚-3-乙酸。
Physical form
0.01 M磷酸盐缓冲液（pH 7.4，含有15 mM叠氮化钠）溶液
Disclaimer
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
T Mori et al.
American journal of reproductive immunology (New York, N.Y. : 1989), 26(3), 97-103 (1991-10-01)
Expression of CD4-like molecule on vitelline membrane of murine eggs was demonstrated by indirect immunofluorescence (IIF) test and immunoprecipitation corresponding to the expression of major histocompatibility complex (MHC) class II molecule on murine sperm detected by immunoblotting. This molecule showed
Manjul Singh et al.
Scientific reports, 7(1), 16101-16101 (2017-11-25)
Plants possess exuberant plasticity that facilitates its ability to adapt and survive under challenging environmental conditions. The developmental plasticity largely depends upon cellular elongation which is governed by a complex network of environmental and phytohormonal signals. Here, we report role
Yue Liang et al.
The Plant cell, 32(11), 3485-3499 (2020-08-28)
Developmental transitions in plants require adequate carbon resources, and organ abscission often occurs due to competition for carbohydrates/assimilates. Physiological studies have indicated that organ abscission may be activated by Suc deprivation; however, an underlying regulatory mechanism that links Suc transport
Yolanda Pérez-Pérez et al.
Frontiers in plant science, 10, 1200-1200 (2019-10-16)
Stress-induced microspore embryogenesis is a model in vitro system of cell reprogramming, totipotency acquisition, and embryo development. After induction, responsive microspores abandon their developmental program to follow an embryogenic pathway, leading to in vitro embryo formation. This process is widely
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International journal of molecular sciences, 24(13) (2023-07-14)
The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present
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