A0856
抗-ATG5（N-末端） 兔抗
affinity isolated antibody, PBS solution
别名:
抗-APG5, 抗-APG5L, 抗-ASP, 抗-凋亡特异性蛋白, 抗ATG5 自噬相关的 5 同源物 （酿酒酵母）, 抗自噬蛋白5样
产品名称
抗-ATG5（N-末端） 兔抗, affinity isolated antibody, PBS solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
PBS solution
mol wt
antigen ~56 kDa (Atg5-Atg12 complex)
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μL
technique(s)
immunofluorescence: suitable
western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
Application
兔抗-ATG5抗体已用于免疫荧光和化学发光蛋白质印迹分析。
兔抗ATG5（N-末端特异性）用于蛋白质印迹分析，以检测通过表达 ATG5 特异性 shRNA 的腺病毒转导后，SK-N-MC 细胞中的 ATG5 水平。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析（1篇论文）
蛋白质免疫印迹分析（1篇论文）
抗 ATG5（N-末端）抗体已被用于：
- 免疫印迹
- 免疫荧光
- 蛋白质印迹
Biochem/physiol Actions
Atg5（Apg5）是一种自噬所需的32 kDa蛋白。Atg5由泛素样修饰剂Atg12共价修饰。由此形成的Atg12-Atg5-Atg16复合物栓系自噬体前体，并调节自噬体的形成。研究报告称通过钙蛋白酶裂解形成的Atg5片段具有促凋亡特性。
兔抗ATG5（N末端）可识别人、大鼠和小鼠的Atg5-Atg12复合物。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途，或人类或动物任何类型的消费或使用。
Immunogen
对应于人ATG5第2-15个氨基酸的合成肽，通过C-末端的半胱氨酸残基与KLH发生偶联。相应的序列在大鼠和小鼠中是相同的。
Physical form
0.01 M 磷酸盐缓冲液（pH 7.4，含有 15 mM 叠氮化钠）溶液
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis
Grootaert M O A J, et al.
Autophagy, 11(11), 2014-2032 (2015)
The deubiquitinating enzyme USP36 controls selective autophagy activation by ubiquitinated proteins.
Emmanuel Taillebourg et al.
Autophagy, 8(5), 767-779 (2012-05-25)
Initially described as a nonspecific degradation process induced upon starvation, autophagy is now known also to be involved in the degradation of specific ubiquitinated substrates such as mitochondria, bacteria and aggregated proteins, ensuring crucial functions in cell physiology and immunity.
Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells
Seto S, et al.
PLoS ONE, 8(12), e86017-e86017 (2013)
Denitsa S Petkova et al.
Viruses, 9(5) (2017-05-23)
Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes
Shintaro Seto et al.
PloS one, 8(12), e86017-e86017 (2014-01-01)
Mycobacterium tuberculosis is an intracellular pathogen that can survive within phagocytic cells by inhibiting phagolysosome biogenesis. However, host cells can control the intracellular M. tuberculosis burden by the induction of autophagy. The mechanism of autophagosome formation to M. tuberculosis has
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持