biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:15,000, dot blot: 1:60,000-1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:80,000
shipped in
wet ice
storage temp.
2-8°C
Application
Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of chicken protein lysates at a dilution of 1:1000 for 1 hour shaking at room temperature. Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of crude supernatant from A. castellanii trophozoites cultures at a 1:2000 dilution for 1 hour in 5% nofat dry milk.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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C Thoma et al.
Gene therapy, 7(12), 1039-1045 (2000-06-29)
The development of efficient and safe methods for in vivo gene transfer is central to the success of gene therapy. Recombinant adenoviral vectors, although highly efficient, are limited by the host immune response, potential safety hazards due to obligatory cotransfer
Michael Hurt et al.
Infection and immunity, 71(11), 6243-6255 (2003-10-24)
The pathogenesis of Acanthamoeba keratitis begins when Acanthamoeba trophozoites bind specifically to mannosylated glycoproteins upregulated on the surfaces of traumatized corneal epithelial cells. When Acanthamoeba castellanii trophozoites are grown in methyl-alpha-D-mannopyranoside, they are induced to secrete a novel 133-kDa protein
B P Schick et al.
Blood, 97(2), 449-458 (2001-01-12)
The serglycin proteoglycan is best known as a hematopoietic cell granule proteoglycan. It has been found that serglycin is synthesized by endothelial cells, is localized to cytoplasmic vesicles, and is constitutively secreted. Serglycin messenger RNA in human umbilical vein endothelial
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