Anti-AP Endonuclease antibody, Mouse monoclonal

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) is a crucial enzyme in the DNA base excision repair (BER) pathway. It recognizes baseless sites in the DNA following spontaneous base loss or removal of a damaged base by different DNA glycosylases. This enzyme is the major AP endonuclease in mammalian cells. The human enzyme shows a high degree of homology with other mammalian AP endonucleases (bovine, mouse, rat, and hamster). APE/Ref1 is a multifunctional enzyme. Its N-terminal region includes a nuclear localization sequence and a redox activity zone, while its AP endonuclease activity resides in the C-terminal region. Redox-factor 1 (Ref1) refers to the reduction/oxidation function of the enzyme.

Monoclonal Anti-AP Endonuclease recognizes human, canine, rat, and mouse AP endonuclease (approx. 37kDa).

The AP endonuclease is a DNA damage repair gene involved in base excision repair.

recombinant human AP endonuclease.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-AP endonuclease antibodies have been used for western blot analysis. The antibody can also be used for microarray, indirect ELISA and immunocytochemistry assays.

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) has a 3′-repair diesterase or phosphatase activity. The major DNA repair activity of the enzyme is nicking the DNA phosphodiester backbone 5′ to the AP site leaving a 3′-hydroxyl group and a 5′-deoxyribose phosphate. APE/Ref1 stimulates the DNA binding activity of many transcription factors (Fos, Jun, NF?B, PAX, HIF1, p53 and CREB), which are involved in cancer promotion and progression. The protein was found at elevated levels in several tumors (ovarian, prostate, cervical and germ cells). Redox-factor 1 (Ref1) maintains transcription factors in their active reduced states.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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