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Merck
CN

A2643

α-淀粉酶 来源于猪胰腺

Type VII-A, DFP treated, ammonium sulfate suspension, ≥500 units/mg protein (E1%/280)

别名:

PPA, al1,4 葡聚糖-4-葡萄糖水解酶,, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶

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UNSPSC Code:
12352204
MDL number:
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type

Type VII-A

form

ammonium sulfate suspension

specific activity

≥500 units/mg protein (E1%/280)

storage temp.

2-8°C

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General description

分子质量:51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链(包含2个SH基团和4个二硫键)以及一个紧密结合的Ca2+ (稳定性所必需的)组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0,最适pH为7。

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 6.1

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

法规信息

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Fushan Liu et al.
Journal of experimental botany, 60(15), 4423-4440 (2009-10-07)
The amylose extender (ae(-)) mutant of maize lacks starch branching enzyme IIb (SBEIIb) activity, resulting in amylopectin with reduced branch point frequency, and longer glucan chains. Recent studies indicate isozymes of soluble starch synthases form high molecular weight complexes with
Buford L Nichols et al.
The Journal of nutrition, 139(4), 684-690 (2009-02-06)
Starch is the major source of food glucose and its digestion requires small intestinal alpha-glucosidic activities provided by the 2 soluble amylases and 4 enzymes bound to the mucosal surface of enterocytes. Two of these mucosal activities are associated with
Phuong Lan Tran et al.
FEBS letters, 588(4), 620-624 (2014-01-21)
The action pattern of Bacillus licheniformis thermostable α-amylase (BLA) was analyzed using a series of (14)C-labeled and non-labeled maltooligosaccharides from maltose (G2) to maltododecaose (G12). Maltononaose (G9) was the preferred substrate, and yielded the smallest Km=0.36 mM, the highest kcat=12.86
Sarika Jain et al.
The American journal of surgical pathology, 37(12), 1824-1830 (2013-11-15)
Chromophobe renal cell carcinoma (ChRCC) and oncocytoma present with a perplexing overlap of morphologic and immunohistochemical features. ChRCC have deletions in the 1p21.1 region including the amylase α-1A gene (AMY1A). No such deletions are found in oncocytoma. Instead, oncocytomas shared
N F Furlan et al.
The Journal of clinical pediatric dentistry, 37(1), 83-87 (2013-01-25)
Anxiety and stress are usually related to the dental treatment situation. The objective was to investigate salivary cortisol and alpha-amylase levels (salivary biomarkers) and heart rate in children undergoing a minor dental procedure (dental prophylaxis). In total, 31 children (range

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