Merck
CN

A2714

Sigma-Aldrich

醛缩酶 来源于兔肌肉

lyophilized powder, ≥8.0 units/mg protein

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别名:
D-果糖-1,6-二磷酸-D-甘油醛-3-磷酸裂解酶, 二磷酸果糖酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

生物来源

rabbit muscle

质量水平

形式

lyophilized powder

specific activity

≥8.0 units/mg protein

组成

Protein, ≥80% biuret

异质活性

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
lactic dehydrogenase ≤0.03%
phosphoglucose isomerase ≤0.6%
pyruvate kinase ≤0.1%
triosephosphate isomerase ≤0.05%

储存温度

−20°C

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应用

醛缩酶用于将 1,6-二磷酸果糖转化为磷酸二羟丙酮和 3-磷酸甘油醛。来自家兔肌肉的醛缩酶已用于 DHAP C3 的立体特异性去质子化 。产品 A2714 基本上不含硫酸盐,含有柠檬酸盐缓冲盐。

生化/生理作用

醛缩酶可参与到糖原异生、卡尔文循环和糖酵解。来自兔肌肉中的醛缩酶属于I类醛缩酶,可形成共价席夫碱中间体。醛缩酶的活性位点在 α/β8 桶型褶皱中央

单位定义

在 pH 7.4、25 °C 条件下,一个单位每分钟将 1.0 μmol 1,6-二磷酸果糖转化为磷酸二羟丙酮和 3-磷酸甘油醛。

外形

基本不含硫酸盐的柠檬酸盐缓冲盐

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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