form
solution
shelf life
40 mo.
color
colorless
application(s)
hematology
histology
storage temp.
room temp
Quality Level
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General description
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Application
酸化的酒精溶液可用于区分退行性苏木精染色。
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
- the prediction of pulmonary metastasis progression in osteosarcoma patients
- the development of multispectral imaging to detect melanin
Biochem/physiol Actions
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Features and Benefits
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
target_organs
Eyes,Central nervous system
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
71.6 °F
flash_point_c
22 °C
法规信息
危险化学品
此项目有
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Multispectral imaging as a tool for melanin detection
Kalleberg, et al.
Journal of Histotechnology, 38(1), 14-21 (2015)
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