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Merck
CN

A3979

Anti-Na+/K+ ATPase (β2-subunit) antibody produced in rabbit

whole antiserum, liquid

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UNSPSC Code:
12352203
MDL number:
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biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

species reactivity

rat

technique(s)

immunohistochemistry: 1:200, western blot: 1:1,000 using brain microsomal preparation

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Atp1b2(24214)

General description

The Na+/K+ ATPase is an integral membrane enzyme that regulates ATP-dependent transport of Na+ and K+ across cell membranes. The Na+/K+ ATPase consists of a large, multipass, transmembrane catalytic α subunit, and an associated smaller β glycoprotein subunit. Studies indicate that there are three isoforms of the α subunit (α1, 2, and 3) and two isoforms of the β subunit (β1 and 2) encoded by two multigene families. Rabbit anti-Na+/K+ ATPase (β2-subunit) antibody is specific for the rat β2-subunit.

Immunogen

β2 subunit fusion protein containing residues 63-285, as deduced from cDNA. The β2 subunit exhibits virtual identity with the glial cell adhesion molecule AMOG.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Rabbit anti-Na+/K+ ATPase (β2-subunit) antibody can be used for western blot (1:1,000 using brain microsomal preparation) and immunohistochemistry (1:200) assays.

Physical form

The antibody is supplied as 200μL of whole antiserum

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A Mobasheri et al.
Cell biology international, 21(4), 201-212 (1997-04-01)
We have used isoform-specific antibodies against the Na+, K(+)-ATPase alpha (alpha 1, alpha 2 and alpha 3) and beta (beta 1 and beta 2) subunit isoforms in order to establish their specific localization in isolated bovine articular chondrocytes. Immunoblotting confirmed
Y Sun et al.
The American journal of physiology, 262(6 Pt 1), C1491-C1499 (1992-06-11)
While Western blot analysis clearly revealed the presence of the alpha- and beta-subunits of Na(+)-K(+)-ATPase in a variety of rat tissues, beta was not readily detectable in liver. This observation was consistent with a previous report indicating that Na(+)-K(+)-ATPase immunoprecipitated
Lylia Nini et al.
BMC biochemistry, 8, 18-18 (2007-09-21)
Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins), composed of G alpha, G beta, and G gamma subunits, are positioned at the inner face of the plasma membrane and relay signals from activated G protein-coupled cell surface receptors to various signaling
Michał Biernacki et al.
Advances in medical sciences, 64(1), 15-23 (2018-09-23)
The effect of chronic administration of [3-(3-carbamoylphenyl)phenyl] N-cyclohexylcarbamate (URB597), inhibitor of fatty acid amide hydrolase (FAAH) that hydrolyzes anandamide, on cross-talk between endocannabinoid system, oxidative status and pro-inflammatory factors in the liver of spontaneously hypertensive rats (SHRs) was investigated. Experiments

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