form
lyophilized powder
extent of labeling
200-400 units per g resin
matrix
acrylic beads
matrix activation
oxirane
matrix spacer
3 atoms
swelling
1 g swells to ~4 mL
storage temp.
−20°C
Application
Used for the purification of biotin containing proteins.
Other Notes
One unit will bind 1.0 μg of d-biotin.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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D C Schriemer et al.
Analytical chemistry, 68(19), 3382-3387 (1996-10-01)
Molecular recognition based on the high affinity of avidin for biotin has been combined with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The rich chemistry of biotinylation reagents underlies the development of a mass tracer method for the rapid and sensitive
E Hazum
Journal of chromatography, 510, 233-238 (1990-06-27)
The avidin-biotin technique has been applied to the purification of gonadotropin releasing hormone (GnRH) receptors from other solubilized membrane proteins. The following steps were involved in this approach: (a) solubilization of rat pituitary GnRH receptor with the zwitterionic detergent CHAPS
Use of avidin-sepharose to isolate and idenify biotin polypeptides from crude extracts.
J A Swack et al.
Analytical biochemistry, 87(1), 114-126 (1978-06-15)
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