生物来源
mouse
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
AP180-I, monoclonal
分子量
antigen 170-180 kDa
包含
15 mM sodium azide
种属反应性
bovine, rat, human
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:10,000 using rat brain extract
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... SNAP91(9892)
mouse ... Snap91(20616)
rat ... Snap91(65178)
一般描述
AP180 binds to clathrin triskelia with a stoichiometry of one per clathrin triskelion (trimer of heavy chains) and thereby induces assembly of clathrin into polyhedral cages. Monoclonal antibodies which react specifically with coated vesicle proteins are useful for studies on the intracellular distribution and structural relationship of clathrin-binding proteins. Mouse monoclonal anti-assembly protein 180 antibody binds to human AP180.
The antibody reacts in immunoblotting of bovine and rat brain preparations with the AP180 polypeptide (180 kDa) and with the proteolytic derivatives of AP180 (approximately 170 kDa). The antibody reacts with neuronal tissues and cultured cell lines. The antibody may be used in immunoaffinity purification. For immunocytochemistry applications, fixation with acetone or methanol is recommended.
免疫原
membrane protein fraction from bovine brain-coated vesicles.
应用
Mouse monoclonal anti-assembly protein 180 antibody can be used for immunoblot and immunofluorescence studies. The antibody can also be used for immunocytochemistry and immunoprecipitation studies.
外形
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
法规信息
新产品
此项目有
S Ahle et al.
The EMBO journal, 5(12), 3143-3149 (1986-12-01)
A clathrin assembly protein (AP180) has been purified and characterized from coated vesicles of bovine brain. This protein has hitherto escaped detection because in SDS-gel electrophoresis it is obscured by the 180 kd heavy chain of clathrin. Despite the similarity
Fangbai Wu et al.
Biochemical and biophysical research communications, 385(2), 247-250 (2009-05-20)
The overproduction and extracellular buildup of amyloid-beta peptide (Abeta) is a critical step in the etiology of Alzheimer's disease. Recent data suggest that intracellular trafficking is of central importance in the production of Abeta. Here we use a neuronal cell
Fangbai Wu et al.
Biochemical and biophysical research communications, 402(2), 297-300 (2010-10-13)
The clathrin-assembly protein AP180 is known to promote the assembly of clathrin-coated vesicles in the neuron. However, it is unknown whether the expression of AP180 is influenced by neuronal activity. In this study, we report that chronic depolarization results in
Catherine M Schwartz et al.
The Journal of comparative neurology, 518(18), 3803-3818 (2010-07-24)
Clathrin-coated vesicles are known to play diverse and pivotal roles in cells. The proper formation of clathrin-coated vesicles is dependent on, and highly regulated by, a large number of clathrin assembly proteins. These assembly proteins likely determine the functional specificity
Aparna Shetty et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(42), 16828-16845 (2013-10-18)
Newly formed synapses undergo maturation during ontogenetic development via mechanisms that remain poorly understood. We show that maturation of the presynaptic endocytotic machinery in CNS neurons requires substitution of the adaptor protein 3 (AP-3) with AP-2 at the presynaptic plasma
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持