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Merck
CN

A4843

抗-FITC− 碱性磷酸酶 兔抗

IgG fraction of antiserum, buffered aqueous glycerol solution

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (i), IHC (p)
Citations:
4
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biological source

rabbit

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues, indirect ELISA: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

FITC (fluorescein isothiocyanate) is a fluorescent dye that is often used to label antibodies in immunofluorescent assays. As FITC does not interfere with the biological action of proteins, it can be used as a hapten to label different proteins. FITC conjugation mainly facilitates the accurate detection and localization of proteins in immunological techniques
Anti-FITC-Alkaline Phosphatase antibody is specific for free FITC and FITC conjugated to various biomolecules such as proteins, oligonucleotides, and nucleic acids.

Immunogen

FITC-BSA

Application

2% paraformaldhyde fixed paraffin embedded human rectal sections were stained with alkaline phosphatase-conjugated rabbit anti-FITC at 1:100.
Anti-FITC-Alkaline Phosphatase antibody is suitable for use in fluorescence in situ hybridization at 1:100 or 1:200 dilutions.

Biochem/physiol Actions

The purified IgG fraction of antiserum is adsorbed to remove any antibodies to BSA and then conjugated to alkaline phosphatase. The conjugate may be used for detection of FITC labeled moieties and for visualization of immunofluorescence by light microscopy.

Physical form

Solution in 0.05 M Tris buffer, containing 1% BSA, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

含少量动物源组分生物产品
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分析证书(COA)

Lot/Batch Number

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Oscar R Lara et al.
Methods in molecular biology (Clifton, N.J.), 295, 301-310 (2004-12-15)
Cell populations can be enriched using a number of physicochemical methods based on characteristics, such as density, size, and volume. Centrifugation using density gradients is typically applied to isolate populations with similar characteristics. Cells obtained using these methods can be
Michael J F Blumer et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 236(8), 2077-2088 (2007-07-13)
In mammals, the exact role of cartilage canals is still under discussion. Therefore, we studied their development in the distal femoral epiphysis of mice to define the importance of these canals. Various approaches were performed to examine the histological, cellular
Romana Illig et al.
RNA (New York, N.Y.), 15(8), 1588-1596 (2009-06-25)
During our study on HOXA13, HOXD12, and HOXD13 mRNA expression in human adult and embryonic tissues, we were confronted with the fact that, within our specimen collection, as in other University Departments in Europe, <20% of all samples yielded reliable
P Heukels et al.
Respiratory research, 19(1), 90-90 (2018-05-12)
Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, however

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