biological source
avidin from egg white
conjugate
ferritin conjugate
form
lyophilized powder
composition
Protein, ~30%
storage temp.
−20°C
General description
Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Application
The product has been used to detect biotin bound to avidin tags in Escherichia coli strains.
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing Tris buffer salt
Preparation Note
Labeled with equine spleen ferritin.
Prepared by modification of the procedure of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Prepared by modification of the procedure of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Analysis Note
Actual ferritin: avidin ratio given on label.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Richard D Woods et al.
Nano letters, 7(6), 1809-1816 (2007-05-11)
We functionalized Escherichia coli FliC flagellin proteins to form tailored nanotubes binding single types or pairs of ligands, including divalent cations, fluorescent antibodies, or biotin-avidin-linked moieties such as ferritins. The ratio of each tag in bifunctionalized flagella could be toggled
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(4), 577-580 (1981-04-01)
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the
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