生物来源
rabbit
偶联物
alkaline phosphatase conjugate
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 27.5 kDa
技术
direct ELISA: 1:400 using using wells coated with 5 μg/mL GST in carbonate buffer
western blot (chemiluminescent): 1:20,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析，以鉴定 GST 融合蛋白的表达。
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
免疫原
日本血吸虫 的重组 GST 在 大肠杆菌中表达 。
应用
Alkaline phosphatase (AP) conjugated anti-GST antibodies have been used as probes for western blot assays. The product can also be used for direct ELISA (1:400).
Purification of GST-SfMannIII from Sf9 insect cells was confirmed by western blot using rabbit anti-GST antibody. Gst-SfMan I was purified from over-expressing SF/SWT-1 cells and ran on SDS-page fell for western blot analysis using rabbit anti-GST as the primary antibody. Alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma) was used as the secondary antibody.
外形
Solution supplied in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
此项目有
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The Journal of biological chemistry, 276(19), 16335-16340 (2001-03-30)
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