A5838
抗 谷胱甘肽-S-转移酶
IgG fraction of antiserum, buffered aqueous solution
别名:
抗 GST
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NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB CL
Citations:
5
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 27.5 kDa
technique(s)
direct ELISA: 1:400 using using wells coated with 5 μg/mL GST in carbonate buffer, western blot (chemiluminescent): 1:20,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析，以鉴定 GST 融合蛋白的表达。
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
Immunogen
日本血吸虫 的重组 GST 在 大肠杆菌中表达 。
Application
Purification of GST-SfMannIII from Sf9 insect cells was confirmed by western blot using rabbit anti-GST antibody. Gst-SfMan I was purified from over-expressing SF/SWT-1 cells and ran on SDS-page fell for western blot analysis using rabbit anti-GST as the primary antibody. Alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma) was used as the secondary antibody.
Alkaline phosphatase (AP) conjugated anti-GST antibodies have been used as probes for western blot assays. The product can also be used for direct ELISA (1:400).
Physical form
Solution supplied in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
此项目有
Jared J Aumiller et al.
Glycobiology, 13(6), 497-507 (2003-03-11)
We have previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little intracellular sialic acid and no detectable CMP-sialic acid. In the accompanying study, we
Qian-Kun Niu et al.
Molecular plant, 6(4), 1091-1108 (2013-05-29)
Pollen germination and embryogenesis are important to sexual plant reproduction. The processes require a large number of genes to be expressed. Transcription of eukaryotic nuclear genes is accomplished by three conserved RNA polymerases acting in association with a set of
Kees-Jan Boogerd et al.
Cardiovascular research, 78(3), 485-493 (2008-02-21)
T-box factors Tbx2 and Tbx3 play key roles in the development of the cardiac conduction system, atrioventricular canal, and outflow tract of the heart. They regulate the gap-junction-encoding gene Connexin43 (Cx43) and other genes critical for heart development and function.
Z Kawar et al.
The Journal of biological chemistry, 276(19), 16335-16340 (2001-03-30)
Previously, we cloned and characterized an insect (Sf9) cell cDNA encoding a class II alpha-mannosidase with amino acid sequence and biochemical similarities to mammalian Golgi alpha-mannosidase II. Since then, it has been demonstrated that other mammalian class II alpha-mannosidases can
Zhao-Xia Ma et al.
PloS one, 10(7), e0132500-e0132500 (2015-07-18)
The Arabidopsis TMS1 encodes a heat shock protein identical to the Hsp40 protein AtERdj3A and plays important roles in the thermotolerance of pollen tubes and other plant tissues. Despite its importance to plant growth and reproduction, little has been known
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