Merck
CN
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文件

A6255

Sigma-Aldrich

α-淀粉酶 来源于猪胰腺

greener alternative

PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

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别名:
PPA, al1,4 葡聚糖-4-葡萄糖水解酶，, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶
EC 号:
3.2.1.1 (BRENDA, IUBMB)
EC 号:
232-565-6
MDL编号:
MFCD00081319
eCl@ss:
32160410

生物来源

Porcine pancreas

质量水平

200

类型

Type I-A

形式

saline suspension

比活

≥1000 units/mg protein (E1%/280)

分子量

51-54 kDa

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

环保替代产品分类

, Enabling

运输

wet ice

储存温度

2-8°C

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相关类别

一般描述

分子质量：51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅《Biofiles》的文章。

应用

α-淀粉酶用于水解α-连接的多糖α键，如淀粉和糖原。产品 A6255 来自猪胰腺，为I-A 型。Sigma 公司生产的α-淀粉酶已被用于各种植物研究，如 拟南芥 代谢研究。

生化/生理作用

α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4) 葡聚糖键。天然底物，如淀粉和糖原，被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白，由约 475 个残基的单一多肽链组成，含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca 2 + 。

单位定义

一个单位将在20℃的pH 6.9下，在3分钟内从淀粉中释放1.0毫克麦芽糖

外形

含 3 mM CaCl 的 2.9 M NaCl 悬液

制备说明

PMSF处理。2×结晶

其他说明

有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

底物

产品编号
说明
价格

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书(COA)

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示例

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How should Product No. A6255, α-Amylase from porcine pancreas, be dissolved?

    The product is soluble in water (1 mg/mL), yielding a clear, colorless solution.

  7. What is the solution stablity of Product No. A6255, α-Amylase from porcine pancreas?

    α-Amylase is stable in 25 mM Tris-HCl, pH 7.5, with 100 mM KCl, refrigerated at near 0 °C or frozen at -20 °C for at least 9 days. Another recommended storage condition is in 1 mM phosphate, pH 7.3, with 30 mM CaCl2 at -15 °C.  

  8. How is Product No. A6255, α-Amylase from porcine pancreas, used?

    α-Amylase is an enzyme that breaks down polysaccharides, such as starch and glycogen, to give glucose and maltose. α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. The α-(1,6) bond is not hydrolyzed.

  9. What is the recommended substrate for the α-Amylase test of Product No. A6255?

    The recommended substrate for the enzymatic α-Amylase test is Product No. S2630, Starch.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Frederick J Warren et al.
Carbohydrate polymers, 117, 192-200 (2014-12-17)
In vitro hydrolysis assays are a key tool in understanding differences in rate and extent of digestion of starchy foods. They offer a greater degree of simplicity and flexibility than dynamic in vitro models or in vivo experiments for quantifiable
Cathrina H Edwards et al.
Nature food, 2(2), 118-126 (2021-10-21)
Positive health effects of dietary fibre have been established; however, the underpinning mechanisms are not well understood. Plant cell walls are the predominant source of fibre in the diet. They encapsulate intracellular starch and delay digestive enzyme ingress, but food
Yogesh Kumar Ahlawat et al.
Frontiers in plant science, 12, 762067-762067 (2021-11-20)
The precise role of KNAT7 transcription factors (TFs) in regulating secondary cell wall (SCW) biosynthesis in poplars has remained unknown, while our understanding of KNAT7 functions in other plants is continuously evolving. To study the impact of genetic modifications of
Joana Pico et al.
Food chemistry, 297, 124990-124990 (2019-06-30)
Use of banana flours as functional ingredients is growing due to their nutritional benefits derived from phenolics and dietary fiber. However, the effect oven-drying, freeze-drying and extrusion on the phenolic compounds or starch digestibility is not understood. In this work
Gael Y F Delamare et al.
Food chemistry: X, 5, 100078-100078 (2020-03-07)
Many carbohydrate foods contain starch that is rapidly digested and elicits a high Glycaemic Index. A legume ingredient (PulseON®) rich in Type 1 resistant starch (RS1) was recently developed; however, its potential as a functional ingredient when processed into a
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实验方案

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

α-淀粉酶酶促测定 (EC 3.2.1.1)

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

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