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化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
Specific activity:
≥1,500 units/mg protein (biuret)
Assay:
≥30%
Biological source:
bacterial (Bacillus amyloliquefaciens)
biological source
bacterial (Bacillus amyloliquefaciens)
type
Type II-A
assay
≥30%
form
lyophilized powder
specific activity
≥1,500 units/mg protein (biuret)
mol wt
50-55 kDa by SDS-PAGE
technique(s)
SDS-PAGE: suitable
solubility
H2O: soluble 0.1 mg/mL, clear, colorless
suitability
suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
application(s)
life science and biopharma
storage temp.
−20°C
Quality Level
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General description
αα-淀粉酶是一种胞外酶,广泛存在于许多动植物中,也分布于微生物中,例如各种芽孢杆菌(Bacillus species)。
α-淀粉酶(1,4-α-d-葡萄糖水解酶),是一种内切葡聚糖酶,属于糖苷水解酶家族13(GH13)的一员。
Application
α-淀粉酶(芽孢杆菌)已用于:
- 作为唾液组分,用于人工咀嚼;胃肠腔消化实验
- 作为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的标准品,测定α-淀粉酶浓度
- 对乙醇不溶物(AIR)脱淀粉,进行非纤维素神经单糖分析
Biochem/physiol Actions
α-淀粉酶可将淀粉降解为麦芽糖等寡糖、葡萄糖和α-极限糊精。它可水解由三个或多个α-(1→4)连接D-葡萄糖单元组成的多糖的α-(1→4)葡聚糖键,无需水解α-(1→6)键。参与葡萄糖生成,对于能量获取至关重要。α-淀粉酶是一种广为人知的工业酶,适用于食品、洗涤、纺织、发酵和制药行业。
Features and Benefits
85 °C,pH 6.2下处理60分钟,依然保持>98%活性。
- α-淀粉酶(来自地衣芽孢杆菌NCIB 6346)在pH 6.2和85°C条件下60分钟后保留98%以上的活性。
- 其他α-淀粉酶在91°C下存放1小时后,仍然具有100%活性。
Preparation Note
4×结晶
以0.1 mg/mL的浓度溶于水,形成透明无色溶液。
Other Notes
在pH 6.9、温度20 °C条件下,一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
本产品仅供研&发使用,不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息,请参阅安全数据说明书。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
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Ajaya K Biswal et al.
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A number of years ago we reported a two-step inactivation mechanism for α-amylase (enzyme) on the basis of theoretical and experimental studies in aqueous solutions. In the first step the metal (Ca2+ ) ion dissociates reversibly from the enzyme followed
实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
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