Merck
CN

A6380

Sigma-Aldrich

α-淀粉酶 来源于芽孢杆菌

Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

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别名:
1,4-α-D-葡聚糖-葡萄糖水解酶
CAS号:
EC 号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

类型

Type II-A

质量水平

形式

lyophilized powder

specific activity

≥1,500 units/mg protein (biuret)

分子量

50-55 kDa by SDS-PAGE

溶解性

H2O: soluble 0.1 mg/mL, clear, colorless

储存温度

−20°C

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一般描述

αα-淀粉酶是一种胞外酶,广泛存在于许多动植物中,也分布于微生物中,例如各种芽孢杆菌(Bacillus species)。
α-Amylase (1,4-α-d-glucan glucohydrolase), an endo-acting glucanase, is a member of the glycoside hydrolase family 13 (GH13).

应用

α-淀粉酶(芽孢杆菌)已用于:

  • 作为唾液组分,用于人工咀嚼;胃肠腔消化实验
  • 作为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的标准品,测定α-淀粉酶浓度
  • 对乙醇不溶物(AIR)脱淀粉,进行非纤维素神经单糖分析

生化/生理作用

α-淀粉酶可将淀粉降解为麦芽糖等寡糖、葡萄糖和α-极限糊精。它可水解由三个或多个α-(1→4)连接D-葡萄糖单元组成的多糖的α-(1→4)葡聚糖键,无需水解α-(1→6)键。参与葡萄糖生成,对于能量获取至关重要。α-淀粉酶是一种广为人知的工业酶,适用于食品、洗涤、纺织、发酵和制药行业。

特点和优势

85 °C,pH 6.2下处理60分钟,依然保持>98%活性。
  • α-Amylase from Bacillus licheniformis NCIB 6346 retains over 98% of its activity after 60 minutes at pH 6.2 and 85°C.
  • Other α-Amylase maintain 100% of their activity after storage for 1 hour at 91°C.

单位定义

在pH 6.9、温度20 °C条件下,一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

制备说明

4×结晶
以0.1 mg/mL的浓度溶于水,形成透明无色溶液。

其他说明

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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Ajaya K Biswal et al.
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Wood cell walls are rich in cellulose, hemicellulose and lignin. Hence, they are important sources of renewable biomass for producing energy and green chemicals. However, extracting desired constituents from wood efficiently poses significant challenges because these polymers are highly cross-linked
Applications of high-hydrostatic-pressure processing on microbial enzymes
Liu Y, et al.
Journal of biological and chemical chronicles, 331-371 (2023)
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Journal of phycology, 44(5), 1221-1234 (2008-10-01)
The cell wall of the green alga Micrasterias denticulata Bréb. ex Ralfs (Desmidiaceae, Zygnematophyceae, Streptophyta) was investigated to obtain information on the composition of component polysaccharides and proteoglycans to allow comparison with higher plants and to understand cell wall functions
Sun-Li Chong et al.
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The atmospheric pressure matrix-assisted laser desorption/ionization with ion trap mass spectrometry (AP-MALDI-ITMS) was investigated for its ability to analyse plant-derived oligosaccharides. The AP-MALDI-ITMS was able to detect xylooligosaccharides (XOS) with chain length of up to ten xylopyranosyl residues. Though the
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ACS applied bio materials, 2(11), 5136-5143 (2019-11-18)
Enzyme-based treatments are used in heritage conservation for the effective removal of glues and other damaging organic layers from the surfaces of historic and artistic works. Despite their potential, however, the application of enzymatic treatments is currently limited because of

实验方案

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

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