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Merck
CN

A6561

Sigma-Aldrich

单克隆抗-生物素

clone BN-34, purified immunoglobulin, buffered aqueous glycerol solution

别名:

Monoclonal Anti-Biotin

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.46

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生物来源

mouse

偶联物

alkaline phosphatase conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

BN-34, monoclonal

表单

buffered aqueous glycerol solution

技术

dot blot: 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil and biotin-monoclonal anti-human IgG
indirect ELISA: 1:30,000 using human IgG and biotin-monoclonal anti-human IgG
western blot (chemiluminescent): 1:10,000 using human IgG and biotin-monoclonal anti-human IgG

同位素/亚型

IgG1

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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一般描述

该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色，该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with biotin-keyhole limpet hemocyanin (KLH).

免疫原

生物素-KLH偶联物

应用

可代替亲和素以降低非特异性结合。
该抗体可被用于扩增技术、ELISA、免疫印迹、原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
Monoclonal Anti-Biotin-Alkaline Phosphatase antibody produced in mouse has been used in:
  • blotting (western), Southern and dot blots)
  • immunohistology
  • in situ nucleic acids hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • fluorescent activated cell-sorting (FACS)
  • electron microscopy

生化/生理作用

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
The high binding affinity of biotin to egg white or bacteria-derived avidin has been exploited in the design of immunoassays and immunohistologic staining techniques. Conventional immunoassay methods are improved using monoclonal anti-biotin, which enhances the sensitivity of avidin-biotin immunoassays by adding a second layer of enzyme via the biotin specific monoclonal antibody.

外形

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
126M4786V
016M4753V
095M4781V
065M4791V
063M4805V

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David E Noga et al.
Biomacromolecules, 9(7), 2056-2062 (2008-06-26)
A polylactide copolymer with pendant benzyloxy groups has been synthesized by the copolymerization of a benzyl-ether substituted monomer with lactide. Debenzylation of the polymer to provide pendant hydroxyl groups followed by modification with succinic anhydride affords the corresponding carboxylic acid
Spatial screening of cyclic neoglycopeptides: identification of polyvalent wheat-germ agglutinin ligands.
Valentin Wittmann et al.
Angewandte Chemie (International ed. in English), 43(7), 900-903 (2004-02-10)
Stereochemical engineering yields a multifunctional peptide macrocycle inhibitor of Akt2 by fine-tuning macrocycle-cell membrane interactions.
Nag, et al.
Communications Chemistry, 6, 95-95 (2023)
Spore surface glycoproteins of Colletotrichum lindemuthianum are recognized by a monoclonal antibody which inhibits adhesion to polystyrene
Hughes H, et al.
Microbiology, 145(8), 1927-1936 (1999)
Yash M Kolambkar et al.
Tissue engineering. Part A, 20(1-2), 398-409 (2013-09-12)
Electrospun nanofiber meshes have emerged as a new generation of scaffold membranes possessing a number of features suitable for tissue regeneration. One of these features is the flexibility to modify their structure and composition to orchestrate specific cellular responses. In
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