A8103
Anti-hABH1 antibody, Mouse monoclonal
clone hABH1-151, purified from hybidoma cell culture
别名:
Anti-Human AlkB Homologue 1, Monoclonal Anti-hABH1 antibody produced in mouse
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关于此项目
Conjugate:
unconjugated
Clone:
hABH1-151, monoclonal
Application:
ARR, ELISA (i), IHC, IP, WB
Citations:
5
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hABH1-151, monoclonal
form
buffered aqueous solution
mol wt
antigen ~45 kDa
species reactivity
human, monkey
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of 293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ALKBH1(8846)
General description
Anti-hABH1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH1-151 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human hABH1, conjugated to keyhole limpet hemocyanin (KLH).
Human AlkB homologue 1 (hABH1) is one of the eight homologs of E. coli AlkB. Homology modeling studies have analyzed the role of hABH1 gene in DNA repair after alkaylation-induced damage.
Application
Anti-hABH1 antibody, Mouse monoclonal has been used in
- chromatin immunoprecipitation(ChIP)
- immunohistochemistry
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
Biochem/physiol Actions
Monoclonal Anti-hABH1 (human AlkB homologue) recognizes human and monkey ABH1. It does not recognize hABH2 or hABH3. The antibody epitope resides within amino acids 19-34 of human hABH1.
The human homolog, AlkB homolog 1, histone H2A dioxygenase (Alkbh1) is linked with several enzymatic actions, such as, DNA, mRNA or tRNA hydroxylation, cleavage at abasic (AP) site in DNA and demethylation of histones. It plays a key role in epigenetic gene silencing, as it is known to exhibit m6A demethylation activity . ALKBH1 participates in normal cell proliferation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
Muller TA, et al.
Biochemical and biophysical research communications, 495(1), 98-103 (2018)
Role of ALKBH1 in the core transcriptional network of embryonic stem cells
Ougland R, et al.
Cellular Physiology and Biochemistry, 38(1), 173-184 (2016)
Shankaracharya et al.
Interdisciplinary sciences, computational life sciences, 3(3), 175-181 (2011-10-01)
Inhibition of DNA repair mechanism through alkylating agents in tumor cells is an important method for cancer treatment. Alkylation damage repair gene AlkB was first reported in E. coli. In human and other mammals eight distinguishing homologs of AlkB were
Rune Ougland et al.
Stem cells (Dayton, Ohio), 30(12), 2672-2682 (2012-09-11)
AlkB homolog 1 (ALKBH1) is one of nine members of the family of mammalian AlkB homologs. Most Alkbh1(-/-) mice die during embryonic development, and survivors are characterized by defects in tissues originating from the ectodermal lineage. In this study, we
Rune Ougland et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 38(1), 173-184 (2016-01-15)
ALKBH1, an AlkB homologue in the 2-oxoglutarate and Fe2+ dependent hydroxylase family, is a histone dioxygenase that removes methyl groups from histone H2A. Studies of transgenic mice lacking Alkbh1 reveal that most Alkbh1-/- embryos die during embryonic development. Embryonic stem
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