Application
Can be used in kinase activity assays, which measure the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF) per minute per mg protein at 30 °C using a final concentration of 50 μM [32P]-ATP.
Biochem/physiol Actions
Akt1 is an isoform of multifunctional Akt kinase, which upon activation becomes involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, cell motility and a number of metabolic effects of insulin.
Physical form
Supplied as a solution at 0.1 mg/ml in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.
Other Notes
GST-tagged human recombinant full length Akt1
One unit will incorporate 1 nanomole of phosphate into the AKT/SGK substrate peptide (RPRAATF) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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Justin Kale et al.
EMBO reports, 19(9) (2018-07-11)
Akt is a pro-survival kinase frequently activated in human cancers and is associated with more aggressive tumors that resist therapy. Here, we connect Akt pathway activation to reduced sensitivity to chemotherapy via Akt phosphorylation of Bax at residue S184, one
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Cervical cancer is the second most common women carcinoma worldwide and the fourth leading cause of cancer-associated mortality in women. Butein, a bioactive flavonoid isolated from numerous native plants, has been shown to induce apoptosis and inhibits migration and invasion
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Pengfei Xu et al.
Molecular and cellular endocrinology, 406, 87-101 (2015-03-01)
White adipose tissue has now been recognized as an important endocrine organ secreting bioactive molecules termed adipocytokines. In obesity, anti-inflammatory adipocytokines like adiponectin are decreased while pro-inflammatory factors are over-produced. These changes contribute to the development of insulin resistance and
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MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in cervical carcinogenesis and progression. microRNA-133a (miR-133a) has been reported to play a tumor-suppressor role in a range of cancers. However, the role and underlying molecular mechanism of miR-133a in
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