A9776
α-Actinin from chicken gizzard
~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension
生物来源
chicken gizzard
方案
~80% α-actinin basis (SDS-PAGE)
表单
ammonium sulfate suspension
分子量
100 kDa
储存温度
2-8°C
基因信息
chicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918)
human ... ACTN1(87)
mouse ... ACTN1(109711)
rat ... ACTN1(81634)
一般描述
α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.
应用
α-Actinin from chicken gizzard has been used:
- as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
- in in vitro motility experiments to test its effect on actin filament movement
- to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies
生化/生理作用
α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.
包装
Package size based on protein content
外形
Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride
制备说明
Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.
Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).
Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
动植物来源生物产品
常规特殊物品
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