SciFlow 1000 Fluidics Culture System

Predict cytotoxic effect while generating more in vivo-like data
The next step in cell-based assays is to reproduce organ and living system complexity for more accurate assessment of adaptive vs. toxic mechanisms of compound treatment. A dynamic exposure scenario allows better prediction of concentration thresholds for response rather than extrapolating from endpoint dose-response curves. To demonstrate the value of this system using high-content imaging, we evaluated the time-course effect of Aflatoxin B on a metabolically competent hepatocyte model using the DNA-binding reagent CellTox^™ Green.

High-throughput imaging of a unique continuous flow microfluidics plate
The continued drive towards cell based assays that better mimic the in vivo environment has led to innovative cell culture systems such as the SciFlow 1000 Fluidic Culture System, which utilizes capillary channels to enable physiologically relevant fluid flow across cultured cells. In this application note, we present the ability to accurately image the wells of this novel culture system with the ImageXpress^® Micro High-Content Imaging Systems to visualize and quantify cytotoxic exposure kinetics.

Serial Multiwell Gradient Exposures for Analyzing Effects of Changing Parent- Metabolite Ratios
The SciFlow Multiwell Cascading Fluidics Cell Culture System is a versatile system that enables the application of fluidic motion and gradient toxicant exposure to cell-based assays in a proprietary microplate reader-compatible 96-well format. Time-resolved dynamic exposure scenarios afforded by the SciFlow System is more in vivo-like and could enable more accurate assessment of adaptive vs toxic mechanisms. Moreover, the dynamic exposure scenario allows better prediction of concentration thresholds for response rather than extrapolating from endpoint dose-response curves.

The SciFlow Multiwell Cascading Fluidics Cell Culture System is a versatile system that enables the application of fluidic motion and gradient toxicant exposure to cell-based assays in a proprietary microplate reader-compatible 96-well format. Time-resolved dynamic exposure scenarios afforded by the SciFlow System is more in vivo-like and could enable more accurate assessment of adaptive vs toxic mechanisms. Moreover, the dynamic exposure scenario allows better prediction of concentration thresholds for response rather than extrapolating from endpoint dose-response curves. The SciFlow System is an SBS formatted microtiter plate designed to be compatible with plate-readers, imagers, and automated liquid handling systems. In order to enable efficient flow of fluids across the plate, the SciFlow System was designed with variable well-heights. The wells are elevated at one end of the plate and subsequently the bottom surface of each well is 0.5mm lower than the previous well. This allows for a cascading flow of fluids across the plate. The Tecan Infinite^® M1000 Pro plate reader features the ability to empirically determine the optimal height of each measurement.

CellTox is a trademark of Promega Corp.

ImageXpress is a registered trademark of Molecular Devices, LLC.

Infinite is a registered trademark of Tecan Group Ltd