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Merck
CN

B21200

Sigma-Aldrich

EnPresso® B Defined Nitrogen-free

Growth system for expressing protein in bacteria, Starter Pack

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UNSPSC代码:
12352200
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无菌性

sterile; γ-irradiated

表单

tablet

pH值(酸碱度)

6.8

运输

ambient

储存温度

room temp

生化/生理作用

EnPresso® B Defined Nitrogen-free 是一种预灭菌,化学成分确定的生长体系,能提高15N标记蛋白的产量。

EnPresso®生长体系可为微生物生长、代谢及蛋白表达提供最佳的培养条件。该体系可达到远超常规培养基的细胞密度,从而提高蛋白得率。通过控制生长速率及代谢环境,可大幅提高表达蛋白的正确折叠比例,提高蛋白产物的溶解率,最大限度降低包涵体的形成,确保蛋白产物的功能。

EnPresso® 生长体系不仅可以维持稳定的pH,提供生长所需的充足矿物质元素、维生素及微量元素,而且可通过专利的EnBase 技术来确保多聚糖基质中的葡萄糖能够稳定、缓慢地释放。

了解EnPresso B 生长体系所有产品的订购信息。

外形

EnPresso® B 以试剂盒形式提供,足够进行4次50 ml 培养。试剂盒组分包括:
4包(蓝色),共8片
1瓶(1 ml)Reagent A

其他说明

BioSilta performance guarantee for EnPresso Starter Packs:
For expression of recombinant proteins in E. coli, BioSilta guarantees a minimun 5-fold increase in protein yield from EnPresso B Defined nitrogen-free growth system when compared to yields from comercially available minimal media. Comparisons must be made using an EnPresso Starter Pack and a commercially-available growth media. Manufacturer′s protocols must be followed exactly. Cultures must be adequately aerated in shake flasks or 24 deepwell plates. Failure to achieve minimum 5-fold increase in yield must be reported to BioSilta (through Sigma Technical Services) within 21 days of product delivery and include quantitative data from the EnPresso growth system and the comparative growth media. Subject to data review, BioSilta/Sigma will refund the purchase price of the EnPresso Starter Pack. Please note this guarantee does not apply to membrane proteins and proteins expressed into the periplasm as performance data is not yet available.

法律信息

EnBase is a trademark of BioSilta Oy
EnPresso is a registered trademark of BioSilta Oy

警示用语:

Danger

危险分类

Aquatic Chronic 2 - Carc. 1A - Carc. 1B Inhalation - Skin Sens. 1 - STOT RE 2

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number

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F Mahboudi et al.
Journal of applied microbiology, 114(2), 364-372 (2012-11-07)
A novel chimeric-truncated form of tissue-type plasminogen activator (t-PA) with improved fibrin affinity and resistance to PAI was successfully produced in CHO expression system during our previous studies. Considering advantages of prokaryotic expression systems, the aim in this study was
Jennifer Jaitzig et al.
ACS synthetic biology, 3(7), 432-438 (2013-12-20)
The structural complexity of nonribosomal peptides (NRPs) impeding economic chemical synthesis and poor cultivability of source organisms limits the development of bioprocesses for novel bioactive compounds. Since nonribosomal peptide synthetases (NRPSs) assemble NRPs from simple amino acid building blocks, heterologous
Thomas Horn et al.
Biochimica et biophysica acta, 1831(12), 1702-1713 (2013-08-21)
Mammalian lipoxygenases belong to a family of lipid-peroxidizing enzymes, which have been implicated in cardiovascular, hyperproliferative and neurodegenerative diseases. Here we report that a naturally occurring mutation in the hALOX15 gene leads to expression of a catalytically near-null enzyme variant
Kristof Zarschler et al.
Microbial cell factories, 12, 97-97 (2013-10-29)
For their application in the area of diagnosis and therapy, single-domain antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments thereof in terms of size, stability, solubility, immunogenicity, production costs as well as tumor uptake and blood clearance. Thus
Kaisa Ukkonen et al.
Microbial cell factories, 12, 73-73 (2013-07-31)
Fab antibody fragments in E. coli are usually directed to the oxidizing periplasmic space for correct folding. From periplasm Fab fragments may further leak into extracellular medium. Information on the cultivation parameters affecting this leakage is scarce, and the unpredictable

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