B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
别名:
protein markers, protein molecular weight markers, protein standards
form
lyophilized powder
Quality Level
usage
vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
mol wt
6,500-180,000 Da
packaging
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
storage temp.
−20°C
Application
对于 SDS-PAGE 和蛋白转移，生物素化分子量标准品混合物含有蛋白，而这些蛋白已与生物素结合，从而用作估算带印迹蛋白分子量的 SDS-PAGE 标准品。使用链霉亲和素-过氧化物酶（编号： S2438 ）和4-氯-1-萘酚（编号：C8890）等显色剂实现显谱，从而避免为一般蛋白染色切出单独通道，且这可与免疫染色程序同时进行。这些标准品可用于 Laemmli 或 Webe和Osborn SDS-PAGE程序。
Preparation Note
用 1 mL 1x 样品缓冲液（产品目录号：S3401）重建每个小瓶，并在100°C培养2-3分钟。
Other Notes
含有 9 种生素蛋白（总计约 0.1 mg）。
本品含有：
- α 2 -巨球蛋白（马血清）
- β-半乳糖苷酶（ 大肠杆菌 ）
- 磷酸化酶 b（兔肌肉）
- 过氧化氢酶（牛肝）
- 乙醇脱氢酶（酵母）
- 碳酸酐酶（牛红细胞）
- 胰蛋白酶抑制剂（大豆）
- 溶菌酶（鸡蛋）
- 抑肽酶（牛肺）
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B2787-1VL | 04061833427101 |
| B2787-5X1VL | 04061833427118 |
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