B3795
脑源性神经营养因子 人
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
别名:
阿立纽林, BDNF
产品名称
脑源性神经营养因子 人, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
mol wt
~27 kDa
packaging
pkg of 10 μg
pkg of 5 μg
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized powder
quality
endotoxin tested
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg Endotoxin level
UniProt accession no.
storage temp.
−20°C
Quality Level
Analysis Note
其生物活性由其刺激人神经母细胞瘤细胞SH-SY5Y增殖的能力来确定。
Application
脑源性神经营养因子（BDNF）重组人蛋白已被用作各种培养基中的补充剂。
Biochem/physiol Actions
脑源性神经营养因子（BDNF）（13.6kDa或27.2kDa二聚体）是神经营养蛋白家族的成员。 BDNF在人、小鼠和猪中具有相同的氨基酸序列，具有完全的交叉反应性。 BDNF在中枢神经系统内的神经元群体或与其直接相关的细胞的发育和维持中具有重要作用。 在体外，BDNF已报到可增强几类神经元的存活和分化，其中包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞。
General description
BDNF是生长因子中神经营养蛋白家族的成员，该家族还包括NGF、NT-3和NT-4。所有神经营养因子具有六个保守的半胱氨酸残基，并且在氨基酸水平上具有55％的序列同一性。BDNF已被证明可以增强体外几类神经元的存活和分化，包括神经嵴和基板衍生的感觉神经元、黑质中的多巴胺能神经元、基底前脑胆碱能神经元、海马神经元和视网膜神经节细胞.1 BDNF在外周神经节内表达，并且不限于神经元靶区域，因而提高了BDNF对神经元以及非神经元细胞具有旁分泌或甚至自分泌作用的可能性。
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
人重组BDNF蛋白（27kDa）的活性形式是由两个相同的119个氨基酸亚基形成的二聚体，它们通过强疏水相互作用结合在一起
Physical form
从经过无菌0.2 μm过滤的BSA溶液中冻干得到
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
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实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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