生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
浓度
1.2 mg/mL
技术
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: suitable
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
BAPTA is a highly selective calcium chelator. BAPTA and its analog BAPTA/AM have been extensively used in cell physiology research for defining the mechanisms underlying inter- or intracellular calcium homeostasis and calcium dependent exocytosis. They may also be used in the study of diseases in which the regulation of excitatory neurotransmitter release and uptake has been disturbed.
免疫原
BAPTA conjugated to KLH.
应用
Anti-BAPTA antibody produced in rabbit is suitable for immunoblotting, immunofluorescence or immunoprecipitation at 1-10 μg/mL. The antibody is used in determining spatial distribution of BAPTA through immunocytochemistry and confocal microscopy of cortical neurons loaded with BAPTA/AM, an analog. It is also useful in determining kinetics of loading and efflux of BAPTA through ELISAs and immunocytochemistry.
外形
Solution in phosphate buffered saline, pH 7.4, with 0.08% sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
法规信息
新产品
此项目有
W M Roberts
Nature, 363(6424), 74-76 (1993-05-06)
The potential importance of intracellular calcium-binding proteins in rapid and highly localized Ca2+ signalling is poorly understood. During fast synaptic transmission, which occurs at specialized active zones where Ca2+ diffuses only a few tens of nanometers from channels to neurotransmitter
M Tymianski et al.
Cell calcium, 22(2), 111-120 (1997-08-01)
In spite of its importance as a tool to manipulate cell calcium, the versatility of the octadentate chelator BAPTA in cell physiological and diverse other applications is limited by the difficulty with which it can be quantified and its cell
Glutamate neurotoxicity and diseases of the nervous system.
D W Choi
Neuron, 1(8), 623-634 (1988-10-01)
D Kline et al.
Developmental biology, 149(1), 80-89 (1992-01-01)
The role of calcium in cortical granule exocytosis and activation of the cell cycle at fertilization was examined in the mouse egg using the calcium chelator BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) and the fluorescent calcium indicator fluo-3. BAPTA and fluo-3 were introduced
商品
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
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