B4287
牛血清白蛋白 来源于牛血清
heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
别名:
BSA, 牛白蛋白, 牛血清白蛋白
生物来源
bovine
质量水平
等级
Molecular Biology
产品线
BioReagent
方案
≥98%
表单
lyophilized powder
分子量
~66 kDa
纯化方式
heat shock fractionation
包装
poly bottle of
来源
USA origin
技术
ELISA: suitable
electrophoresis: suitable
western blot: suitable
杂质
protease, free
pH值（酸碱度）
7
溶解性
water: soluble (40 mg/ml)
UniProt登记号
异质活性
Protease free
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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应用
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特点和优势
- Protease free
- Suitable for blocking non-specific protein binding sites in hybridizations
- Heat shock fractionated
制备说明
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Robert W Crawford et al.
Infection and immunity, 76(11), 5341-5349 (2008-09-17)
Salmonella enterica serovar Typhi can establish a chronic, asymptomatic infection of the human gallbladder, suggesting that this bacterium utilizes novel mechanisms to mediate enhanced colonization and persistence in a bile-rich environment. Gallstones are one of the most important risk factors
Eva P Browne et al.
Aquatic toxicology (Amsterdam, Netherlands), 92(4), 281-287 (2009-04-07)
Polybrominated diphenyl ethers (PBDEs) are brominated flame retardants that persist in the environment and are present in geographically widespread fish species. PBDE concentrations can be particularly high in resident Chinook salmon (Onchorhynchus tshawytscha) in the Puget Sound, Washington. Although PBDE
Ruth Jinfen Chai et al.
PloS one, 6(12), e28090-e28090 (2011-12-14)
Reasons for the progressive age-related loss of skeletal muscle mass and function, namely sarcopenia, are complex. Few studies describe sarcopenia in mice, although this species is the mammalian model of choice for genetic intervention and development of pharmaceutical interventions for
实验方案
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
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