B4287
牛血清白蛋白 来源于牛血清
heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
别名:
BSA, 牛白蛋白, 牛血清白蛋白
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关于此项目
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable
electrophoresis: suitable
western blot: suitable
electrophoresis: suitable
western blot: suitable
Impurities:
protease, free
产品名称
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
biological source
bovine
grade
Molecular Biology
product line
BioReagent
assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable
electrophoresis: suitable
western blot: suitable
impurities
protease, free
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
Protease free
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... ALB(280717)
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Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Preparation Note
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
Application
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
Features and Benefits
- Protease free
- Suitable for blocking non-specific protein binding sites in hybridizations
- Heat shock fractionated
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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实验方案
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
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