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Merck
CN

B6901

Sigma-Aldrich

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

别名:

Monoclonal Anti-Bovine IgG

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.46
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生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

secondary antibodies

克隆

BG-18, monoclonal

包含

15 mM sodium azide

种属反应性

bovine (non-reactive to IgM)

请勿与下列物质发生反应

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

技术

dot blot: suitable
indirect ELISA: 1:1000

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

免疫原

Purified bovine IgG

应用

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
bovine IgG1 and IgG2

外形

Ascites fluid containing 0.1% sodium azide

制备说明

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Clare F J Grant et al.
Journal of virology, 91(9) (2017-02-24)
Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease. Antibodies are pivotal in providing protection against FMDV infection. Serological protection against one FMDV serotype does not confer interserotype protection. However, some historical data have shown that interserotype protection can
Histological and Immunohistochemical Studies on the Furstenberg's Rosette in Cows.
Asti R N, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 17(2), 520-520 (2011)
B Gottstein et al.
International journal for parasitology, 28(4), 679-691 (1998-05-29)
Neospora caninum has gained considerable attention through its role in the aetiology of bovine abortion. Due to its close phylogenetic relationship with Toxoplasma gondii, respective unequivocal differential diagnosis deserves special consideration. In order to evaluate the diagnostic performance of molecular
Mojtaba Porbahaie et al.
Nutrients, 14(21) (2022-11-12)
Bovine milk IgG (bIgG) was shown to bind to and neutralize the human respiratory synovial virus (RSV). In animal models, adding bIgG prevented experimental RSV infection and increased the number of activated T cells. This enhanced activation of RSV-specific T
Chika Okafor et al.
Sensors (Basel, Switzerland), 8(9), 6015-6025 (2008-09-26)
Johne's disease (JD) is one of the most costly bacterial diseases in cattle. In the U.S., economic losses from the disease have been estimated to exceed $1,500,000,000 per year, mainly from the effects of reduced milk production. Current diagnostic tests

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