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Merck
CN

B6901

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

别名:

Monoclonal Anti-Bovine IgG

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关于此项目

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
BG-18, monoclonal
Application:
DB, ELISA (i)
Citations:
12
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BG-18, monoclonal

contains

15 mM sodium azide

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

dot blot: suitable, indirect ELISA: 1:1000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
bovine IgG1 and IgG2

Physical form

Ascites fluid containing 0.1% sodium azide

Preparation Note

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动物来源生物产品
常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Clare F J Grant et al.
Journal of virology, 91(9) (2017-02-24)
Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease. Antibodies are pivotal in providing protection against FMDV infection. Serological protection against one FMDV serotype does not confer interserotype protection. However, some historical data have shown that interserotype protection can
Chika Okafor et al.
Sensors (Basel, Switzerland), 8(9), 6015-6025 (2008-09-26)
Johne's disease (JD) is one of the most costly bacterial diseases in cattle. In the U.S., economic losses from the disease have been estimated to exceed $1,500,000,000 per year, mainly from the effects of reduced milk production. Current diagnostic tests
Ulrike I Mödder et al.
The Journal of biological chemistry, 284(28), 18767-18777 (2009-05-09)
Both estrogen receptor (ER) and peroxisome proliferator-activated receptor gamma (PPARgamma) regulate bone metabolism, and because steroid receptor coactivator (SRC)-2 (TIF-2) enhances ER and PPARgamma activity, we examined the consequences of deletion of SRC-2 on bone using SRC-2 knock out (KO)



全球贸易项目编号

货号GTIN
B6901-.2ML04061837514876