fluorescence
λex 546 nm; λem 579 nm
shipped in
wet ice
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General description
Baseclick EdU HTS Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU(5-乙炔基-2'.-脱氧尿苷)是一种胸苷核苷类似物,在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同,EdU HTS Assay不依赖抗体,因而不需进行DNA变性来检测掺入的核苷。恰恰相反,EdU HTS Kit利用点击化学法来检测不同种类的染料荧光示值。此外,该试剂盒的实验方案经过简化,减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成,在结果的内容和情境丰富程度方面,堪比多重分析方法。
Application
高通量筛选
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C
supp_hazards
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
商品
Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.
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